Progesterone Mediates its Anti-Mitogenic and Anti-Apoptotic Actions in Rat Granulosa Cells Through a Progesterone-Binding Protein With Gamma Aminobutyric AcidA Receptor-Like Features1

Progesterone mediates its anti-mitogenic and anti-apoptotic actions in rat granulosa cells through a progesterone-binding protein with gamma aminobutyric acidA receptor-like features.

Progesterone (P4) inhibits small granulosa cell (GC) mitosis and large GC apoptosis. These actions are steroid specific and dose dependent and are inhibited by the progesterone receptor (PR) antagonist, RU-486. However, these cells do not express the nuclear PR but rather an ill-defined P4-binding protein (P4BP). This binding protein could function as a receptor and mediate P4's actions in GCs....

Progesterone receptor-mediated effects on apoptosis in periovulatory granulosa cells

Orexin A promotes granulosa cell secretion of progesterone in sheep

Background: Orexin A, a small-molecule peptide, can regulate female hormones, but limited evidence for its mechanism of activity exists in ovine. Aims: The objective of this study was to investigate the effect of orexin A on progesterone (P4) secretion in cultured granulosa of sheep follicles. Methods: Sheep ovarian granulosa were isolated and ...

Decreased Benzodiazepine Binding with Little Effect on g-Aminobutyric Acid Binding in Rat Brain After Treatment with Antisense Oligodeoxynucleotide to the g-Aminobutyric AcidA Receptor Gamma-2 Subunit

Benzodiazepine potentiation of g-aminobutyric acid (GABA) neurotransmission is associated with the presence of a gamma-2 subunit in the GABAA receptor. A method was developed to modify the gamma-2 subunit expression in adult rat brain. Unilateral intracerebroventricular (i.c.v.) infusion of a 17-base phosphorothioate-modified antisense oligodeoxynucleotide (ASO) was performed every 12 hr for 3 ...

Evaluation of [18F]FPTT Molecular structure and its binding to progesterone receptor (PR) for PET scan of breast cancer FPTT Molecular structure and its binding to progesterone receptor (PR) for PET scan of breast cancer

Breast cancer is a complicated disease that it is accompanied by different symptoms. Diagnosis of this disease is performed by various techniques. Using Radiopharmaceuticals is a new method to diagnose the said tumors. [18F]-FPTT is one of these nuclear medicines for detection of breast cancer. It seems that the binding of the title radiopharmaceutical to the progesterone receptor is...